ETILTEST
SALIVA ALCOHOL TEST

Rapid Test Etil Test is an enzyme test for the quick and safe detection of alcohol (alc) (ethyl alcohol / ethanol) in saliva and other liquid samples. By analyzing the saliva sample it is possible to determine the concentration of alcohol in the blood.
With this enzymatic analysis a qualitative result is obtained, i.e. the test shows the presence or absence of alcohol in the samples.
The assay detects an ethanol concentration of 0.1 or 10 mg / dL, which is displayed by a light green-gray tint of the Reactive Buffer or Test Buffer. The green becomes more intense as the alcohol concentration in the sample increases. Therefore, in the presence of alcohol in the sample, Etil Test determines a color change that goes from light green-gray in the presence of an alcohol concentration of 0.1, passing to a slightly more intense green with an average concentration, until it assumes a color. dark green-gray if the alcohol concentration approaches 1.50.
We recommend using this visual test only as a qualitative test, as the discrimination ability of the human eye can lead to errors in terms of quantitative results.
It measures 5 levels of alcohol in the body: 0.00 g / l - 0.20 g / l - 0.50 g / l - 0.80 g / l -1.50 g / l.
The Ethyl Test consists of a plastic stick whose end is covered with a reactive pad. The pad, in contact with the alcohol solutions, quickly dyes with shades from green to blue, depending on the quantity of alcohol contained in the liquid. The reactive buffer reacts on the basis of a solid phase chemical synthesis.

How to use
1. Do not put anything in the mouth within fifteen (15) minutes prior to the start of the test. This warning includes non-alcoholic beverages, tobacco products, coffee, breath mints, food, etc.
2. Bring the sealed pouch to room temperature (15-30 ° C) to avoid condensation of moisture on the reactive pad.
3. If you are analyzing samples of liquids other than saliva, make sure those liquids are at room temperature before testing.
4. Open the package and remove the stick. Observe the test pad located on the end of the stick. The swab should be light cream. A stick with a dark brown or stained reactive swab should be discarded.
5. Impregnate the reactive swab with the liquid or saliva taken from the mouth or from a spittoon. Start the stopwatch immediately.
6. After two (2) minutes, observe the color change (if it changes) on the Reactive Pad. If the swab turns green or dark green-gray, the test has detected the presence of alcohol and the result is positive. The results obtained after more than 3 minutes may be wrong.
7. Estimate the approximate blood alcohol concentration by comparing the color of the reactive swab with the color scale on the test package. We recommend using the visual test only as a qualitative test.

Interpretation of the results
After 2 minutes, read the color of the reactive pad. Do not compare the test result with other color scales. Use only the color scale shown on the package. The reaction occurs more slowly in saliva than in other aqueous solutions.

Limits
If, after introducing food, drink or other items into the mouth, you do not wait at least 15 minutes before carrying out the test, the results may be incorrect due to the possible contamination of the saliva with interfering substances.
Etil Test has been designed and calibrated to be interpreted 2 minutes after soaking the reactive pad. If you wait more than 2 minutes, the highlighted results may be wrong or you may have false positive results.
Ethyl Test can be used to detect the presence of alcohol in fluids other than saliva. However, when used in this way, the color scale shown on the package cannot be used as a reference.
If alcohol is present in the fluid, the color of the pad will take on a color ranging from light green-gray to black or cocoa as the alcohol concentration increases. If the swab is immersed in pure alcohol, the swab will discolour slightly or will not stain at all, due to the absence of water that is required for the staining reaction to take place.
When analyzing drinks, the result can only be considered positive if the swab turns very dark brown.
Etil Test is a very sensitive test to alcohol. Alcoholic vapors often present in the air are sometimes detected by the Ethyl Test.
Alcohol vapors are often present in many public places and inside homes. Alcohol is found in many household cleaning products such as disinfectants, deodorants and window cleaners. If alcohol vapors are suspected, testing should be done in an area where these vapors are not present (outdoors, for example).
Note: The person who will read the test results cannot be color blind.

Checks
It is possible to qualitatively check the integrity of the EthylTest, using a solution prepared by adding 4 drops of distilled liqueur at 40% alcohol by volume to a glass of water.
With this solution, the staining should correspond to the window of 0.04% or greater (darker).
The color reaction with alcohol in saliva occurs more slowly and with a less intense color than with alcohol in aqueous solutions.

Characteristics
Specificity: Ethyl test will react in the presence of methyl, ethyl and allyl alcohol. Etil Test will not produce any reaction with alcohols having 5 or more carbon atoms, nor with glycine, glycerol or serine.
This property derives from the specificity of the alcohol oxidase enzyme extracted from the yeast.

Interactions
The following substances may interfere with the Ethyl Test Stick if liquid samples other than saliva are used:
Dye-intensifying agents:
- Peroxide.
- Strong oxidants.
- (common in detergents, pickling and whitening substances).
Agents that inhibit staining:
Reducing agents:
- Ascorbic acid.
- Tannic acid.
- Pyrogallol.
- Mercaptans and tosylates.
- Oxalic acid.
- Uric acid.
- Bilirubin.
- L-dopamine.
- L-methyldopamine.
- Metampirone.
The above substances are usually not present in saliva in sufficient quantities to interfere with the test. However, they must not be placed in the mouth 15 minutes before the test.

Warnings
- Single use.
- Product not intended for medical or diagnostic purposes.
- Do not use after the expiration date.
- Allow the reactive pad to absorb a quantity of liquid such as to completely impregnate it.
- Do not eat, drink or smoke while the samples or kit are being handled.
- Do not use the test if the bag is damaged.
- Bring the reagents to room temperature (15-30 ° C) before use.
- Do not touch the reagent pad on the stick to avoid contaminating it.
- Avoid contaminating the samples with each other, using a new collection container for each sample.
- Not to be used for medical or diagnostic use.
- Handle all specimens as if they contain infectious agents. During testing, observe precautions against microbiological hazards and follow standard procedures for proper disposal of specimens.
- Leave the stick in the sealed bag until use.
- Observe the test result after 2-3 minutes.
- Humidity and high temperatures can affect the test results.
- Do not perform the test in an atmosphere containing alcohol.
- Pure alcohol can give false results.
- Pay attention to any cross-reactivity.
- We recommend the use of the visual test only as a qualitative test.
- The person reading the results cannot be color blind.

storage
The test kit should be stored refrigerated or at room temperature (2-30C °) in the sealed pouch, depending on the shelf life of the product.
Bring the stick to room temperature to avoid condensation of moisture on the reactive buffer.

Composition / Information on ingredients
Complete composition of the kit:
- Test Strip: Contains a filter paper pad with alcohol oxidase, peroxidase, 3,3,5,5-tetramethylbenzedine (TMB) and dry TRIS-HCl buffer.
- The test strip consists of: PE catalyst, reagent buffer, filter paper.
- Other components: aluminum bag, hygroscopic silica gel.
Harmful components:
nobody.

Format
Pack of 1 piece.

Code CALC1S