Description
Membrane immunochromatographic test using highly sensitive monoclonal antibodies to detect the nucleocapsid protein from SARS-CoV-2.
The test strip consists of the following parts: sample buffer, reagent buffer, reaction membrane and absorbent buffer. The reagent buffer contains the orocolloid conjugated with the monoclonal antibody against the SARS-CoV-2 nucleocapsid protein; the reaction membrane contains the secondary antibodies to the SARS-CoV-2 protein nucleocapsid. The whole strip is fixed inside a plastic device.
When the sample is added to the sample well, the conjugates absorbed in the reagent buffer are dissolved and migrate with the sample.
If SARS-CoV-2 antigen is present in the sample, the SARS-CoV-2 antibody conjugate complex and virus will be captured by the anti-SARS-CoV-2 specific monoclonal antibodies coated on the test line region (T).
The absence of the T line suggests a negative result.
To serve as a procedural control, a red line will always appear in the control line region (C) indicating that an appropriate volume of sample has been added and that the membrane absorption effect has occurred.
The test is suitable for people with COVID-19-like symptoms, such as cough, fever, fatigue, etc., to assist in the early detection of SARS-CoV-2 infection. It can also be used to examine people without COVID symptoms -19 to regularly monitor their state of health.

How to use
Preparation before the test
Place the test kit components at room temperature (15-30 ° C).
Blow your nose to clear the nasal passages. Wash your hands and keep them dry.
Read the instructions for use carefully.
Check the expiration date on the package. Do not use expired tests.

Test procedure
Open the sample buffer package. Do not touch the tip of the swab.
Gently insert the tip of the swab into a nasal cavity, to a depth of 2-3 cm. Gently rotate the swab 5 times for 20 seconds to collect the secretion from the nose.
Do not force the tampon so as not to injure the nose.
Gently insert the same swab into the other nasal cavity and repeat the same procedure.

Remove the seal from the extraction tube and insert the swab into the extraction tube. Mix the swab in the solution at least 5 times.
Squeeze the tube with your fingers. Move the swab up and down at least 3 times to expel any sample solution from the swab.
Pull out the swab. Firmly insert the cap onto the extraction tube. Gently squeeze the tube 3 times to mix the sample solution. Let the tube rest for 1 minute.

Open the foil pouch containing the test card and place it on a table.
Invert the extraction tube and add 3 drops of sample solution to the loading well (S) on the test card.
Read the result after 15 minutes. The result is considered inaccurate and invalid after 30 minutes.
Do not refill the sample solution on a used test card.

After interpretation of results, use household bleach spray or a 70-75% alcohol spray to disinfect used components of the product. Put the used components in a plastic bag. Close the bag and put it in another plastic bag. Dispose of the bag with household waste.
Wash your hands thoroughly.

Interpretation of results
Positive (+): Red bands appear on both the T and C lines in 15